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. 2015 Jul 16;6(7):e1818. doi: 10.1038/cddis.2015.182

Figure 7.

Figure 7

Aip1 deletion in Sertoli cells resulted in increase of spermatogonial differentiation and decrease of SSC self-renewal. (a) Co-immunostaining of OCT4 and PLZF in P4, P7 and P9 seAip1−/− and control testes. The result was quantified in (d). (b) Co-immunostaining of MVH and PLZF in P7, P9 and P12 seAip1−/− and control testes, with results quantified in (e) and (f). (c) Co-immunostaining of activated caspase-3 and PLZF in P7, P9 and P12 seAip1−/− and control testes, with results quantified in (g). (d–g) Quantification of SSC (d; PLZF and OCT double positive), undifferentiated spermatogonia (e; labeled by PLZF), germ cells (f; labeled by MVH) and apoptotic spermatogonia (g; PLZF and activated caspase-3 double positive) per cross-section of each testicular cord for both seAip1−/− and control mice at indicated postnatal dates. A total of 51 tissue cross-sections were analyzed for each genotype at a certain time point (from three mice, except for P12 in c and g). (h and i) Relative expression levels of c-Kit (h) and Stra8 (i) from RT-PCR analysis. (j and k) Increased differentiation as demonstrated by flow cytometry analysis of the c-Kit+ cells. (j) Quantification of percentage of c-Kit+ cell from flow cytometry data. (k) Scale bars: 50 μm. Data are presented as means±S.E.M.; *P<0.05, **P<0.01, ***P<0.001