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. 2015 Jul 16;6(7):e1818. doi: 10.1038/cddis.2015.182

Figure 8.

Figure 8

SSCs transplanted from defective niche maintained stem cell function and spermatogenesis potential both in vivo and in vitro. To trace the donor germ cells, especially in transplantation assay, seAip1−/− mice were crossed to Rosa26-mT/mG mice, a dual color reporter line, which possesses loxP sites on either side of a membrane-targeted tdTomato (mT) cassette. After breeding with cre transgenic mice, the resulting offspring would have the mT cassette deleted and the downstream membrane-targeted EGFP (mG) cassette expressed. (a) Representative THY1+ germ cell clumps from testes of seAip1−/−:Rosa26-mT/mG and Aip1fl/fl:Rosa26-mT/mG mice that had been passaged for 16 times (2.5 months). (b, c) Representative images of recipient testes containing mT-labeled colonies (b) and mT-labeled seminiferous tubules from recipient testes (c). (d) Normal spermatogenesis shown by HE staining of the recipient testis that was transplanted with cells from seAip1−/−:Rosa26-mT/mG and Aip1fl/fl:Rosa26-mT/mG. Scale bars: 10 μm in (a), 1 mm in (b), 0.2 mm in (c) and 20 μm in (d)