Figure 3.
Induction of BMF sensitizes the release of BIM from BCL-XL. (a) MDA-MB-468 cells were transduced with non-targeting shRNA (NS) or p53si-1 lentiviral shRNA for 72 h and the interaction of BMF/BCL-XL and BIM/BCL-XL was detected by co-immunoprecipitation assay. Inputs for co-immunoprecipitation were also subjected to immunoblot analysis. IgG and heavy chain (HC) were used as a negative control and loading control, respectively. (b–c) Overexpression of BCL-XL abrogated the apoptotic effects of mutant p53-R273H knockdown in MDA-MB-468 cells. Cells were co-transfected with BCL-XL expression construct and p53si-1 shRNA. Protein lysates and apoptosis were analyzed by immunoblotting and annexin V/7-AAD flow cytometry at 72 h after co-transfection. Bars represent mean±S.D. of three experiments. *indicates statistical significance (P<0.05) by Student's t-test as compared with vector control cells