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. 2015 May 14;5:10311. doi: 10.1038/srep10311

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Two-photon luminescence (TPL) of GRBs were measured using three different breast cancer cell lines SKBR-3, BT-549, MCF-7 and compared with MCF-10A, breast epithelial cells (A) Light micrscopy images of SKBR-3, MCF-10A, BT-549, and MCF-7 cell lines using the phase contrast mode after GRBs internalization in both passive and active targeting (B) TPL images of the same cell lines, which clearly indicated SKBR-3 cells exhibit maximum photoluminescence with active targeting whereas nonspecific adsorption was observed in control MCF-10A cells.