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. 2015 May 11;5:10115. doi: 10.1038/srep10115

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Copyright © 2015, Macmillan Publishers Limited

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Mutation of the HxD-histidine in the tumor suppressor LKB1 kinase impaired its activity and the ability to suppress anchorage-independent growth. A549 cell lines over-expressing WT, K87I (KD mutant), or H174R LKB1 were analyzed for their ability to form colonies in agarose (a-d). The average numbers of colonies per six-well plate (N = 3) were counted after 14 days. The error bars represent the S.E.M. Significance was analyzed using a two-tailed unpaired Student t test. A value of p < 0.01 (^**) was considered highly statistically significant. (e) Immunoblotting analysis of the active LKB1-AMPK pathway by detection of phospho-AMPK and phospho-ACC in A549 cells over-expressing WT LKB1, H174R, or K87I mutants. Representative results of three independent experiments are shown.