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. 2015 Feb 9;5:8317. doi: 10.1038/srep08317

Figure 1. Generation of photosensitive C2C12 myotubes.

Figure 1

(A) Time schedule for the OS training assay with C2C12 myotubes. One day after seeding (Day −1), cells were transfected with ChRGR-Ve in the growth medium (GM). Twenty-four hours later (Day 0, D0), the medium was replaced with differentiation medium (DM) to promote the formation of multinuclear myotubes. The OS training assay was performed on Days 7–10. (B) Generation of ChRGR-Ve-expressing multinucleated myotubes on day 0 (D0), day 1 (D1), day 4 (D4), and day 7 (D7) after switching to DM. Multinucleated myotubes expressing ChRGR were detected based on Venus-fluorescence (green), and the cell nuclei were stained with Hoechst 33258 (blue). Scale bar, 50 μm.