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. 2015 Nov 6;7:ecurrents.hd.b966ec2eca8e2d89d2bb4d020be4351e. [Version 1] doi: 10.1371/currents.hd.b966ec2eca8e2d89d2bb4d020be4351e

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Fig4rb — A-B. Lentivirus encoding N171 htt fragments was injected intra-striatally into 8-week-old mice; sacrifice was at 14-weeks of age. A. Detection of N171 htt in mouse striata following stereotaxic injection. Brains sections at the level of the stereotaxic injections were stained for N171 htt using MAB5492 (Chemicon) and with a fluorescent Nissl staining. Top left shows detection of 171-18Q; top right shows detection of N171-82Q; bottom show fluorescent Nissl staining. B. Detection of htt expression by Western blot analysis. Striata were dissected then homogenized to extract protein for Western blot analysis using MAB5492. N171-18Q is not detected. N171-82Q is detected.