Fig. 5.

Bone targeting of Cy7-conjugated BP NPs. Mice were treated with 100 µL of 0.1 mg/mL of Cy7-conjugated BP NPs. a NIR fluorescent images of the bones extracted from mice treated with cy7-conjugated BP NPs and control NPs. b Histogram of the fluorescence of the bones treated with cy7-conjugated BP NPs and control NPs. Spectrally unmixed, high contrast bone images were obtained and fluorescent intensity measurements was performed using ImageJ software