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. 2015 Nov 17;16:27. doi: 10.1186/s12860-015-0073-5

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© Ponti et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — B23 levels are directly correlated to EGR1 under serum deprivation. EGR1 and B23 expression in HeLa cells transfected with a GFP fused full length EGR1 (named EGR1-GFP) or incubated with siRNA specific for EGR1 (named siRNA EGR1). A control empty vector for GFP (named GFP) or a scrambled sequence of the siRNA EGR1 (named siRNA SCRAMBLED) were used for comparison, respectively. Experiments were carried out in medium with either 0.2 % or 10 % FCS. Relative levels of B23 and EGR1 mRNA were quantitated by RT-qPCR in cells transfected with EGR1-GFP or control GFP (Panel a), and in cells incubated with siRNA EGR1 or control siRNA scrambled (Panel d). Protein signal ratios of EGR1 and B23 to GAPDH were quantitated by densitometry in cells transfected with EGR1-GFP or control GFP (Panel c), and in cells incubated with siRNA EGR1 or control siRNA scrambled (Panel f), limitedly to serum deprived conditions. Immunoblot of EGR1 and B23 proteins from a representative experiment (Panel b and e). Data are the mean +/− s.e. of three independent experiments. Comparisons were performed by t-test. Significant results are highlighted with asterisks (*p < 0.05; **p < 0.01; ***p < 0.001)