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. 2015 Jun 12;5:11295. doi: 10.1038/srep11295

Figure 1. Molm-14 Engraft in NSG Mice and Contribute to Compartment Exosome Secretion.

Figure 1

(a) Immunohistochemistry of Molm-14-engrafted NSG femurs. Femurs were collected from Molm-14-engrafted mice at 3 weeks post-engraftment, sectioned, and stained for human CD45. (b) PCR identification of NSG-engrafted AML. Molm-14 isolated from engrafted NSG mice retained their characteristic FLT3-ITD mutation, detected by 40 cycles of PCR (cropped; full gel in Figure S2). NTC: null-template control; NSG-AML: isolated Molm-14; HL-60: cell line exhibiting WT FLT3; M14: Molm-14 stock culture. (c,d) Transmission electron microscopy of exosomes. Exosomes were isolated from the culture media of Molm-14 and NSG stroma, and examined by transmission electron microscopy. Sizing of more than 100 vesicles per sample is presented in (c); representative stromal exosome image is presented in (d).