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. 2015 Nov 14;34:140. doi: 10.1186/s13046-015-0255-1

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© Mileo et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — HCV core protein expression in HuH-7 stable transfectants. a Map of pcCAG39EFneo, the mammalian expression vector for cloning and expressing HCV core protein fragment (nt 1-191). b Western Blot detection of the HCV core protein constitutively expressed in HuH-7-CORE cell lysates. β-actin detection in the same blot is shown as loading control. c Immunofluorescence of the HCV core protein expression in HuH-7-CORE cell lines showing its peri-nuclear localization