Investigating a role for β-adrenoceptors in TRPV1 antagonist-induced hyperthermia. A) Core body temperature recordings over 300 min following pretreatment with metoprolol (20 mg/kg, s.c.; n = 6) or control (saline, 5 ml/kg, s.c.; n = 13). B) The hyperthermic index was analyzed as area under the temperature curve during the 0 to 180 min period for saline and metoprolol-pretreated mice. C) Core body temperature recordings over 300 min following pretreatment with ICI-118,551 (10 mg/kg, s.c.; n = 6) or control (saline, 5 ml/kg, s.c.; n = 13). D) Respective hyperthermic index calculated for (C). E) Core body temperature recordings over 300 min following pretreatment with SR59230A (2.5 mg/kg, s.c.; n = 3) or control (2% DMSO in saline, 5 ml/kg, s.c.; n = 4). Arrows in (A), (C), and (E) denote treatments. Results are means ± sem. **P < 0.01, and ***P < 0.001 vs. vehicle control; ##P < 0.01 vs. AMG9810-treated mice using 2-way ANOVA and Bonferroni’s post hoc test.