TABLE 2.

Relative mRNA expression of thermogenic mediators in AMG9810 or vehicle-pretreated TRPV1 WT and KO mice

Target	Tissue	WT vehicle	WT AMG9810	KO vehicle	KO AMG9810
UCP1	BAT	1.03 ± 0.18	0.50 ± 0.11	0.26 ± 0.06	0.12 ± 0.11
	GM	1.09 ± 0.28	0.94 ± 0.21	0.65 ± 0.10	0.92 ± 0.18
UCP2	BAT	1.00 ± 0.03	1.31 ± 0.42	0.68 ± 0.12	0.11 ± 0.02
	GM	0.91 ± 0.69	0.62 ± 0.18	0.49 ± 0.21	0.70 ± 0.21
UCP3	GM	1.03 ± 0.17	0.63 ± 0.07	0.94 ± 0.04	0.44 ± 0.03
	GM	0.63 ± 0.01	1.11 ± 0.36	0.48 ± 0.23	0.70 ± 0.12
PGC1-α	BAT	0.69 ± 0.16	2.07 ± 0.48**	0.97 ± 0.22	0.61 ± 0.10##
	GM	1.02 ± 0.16	0.74 ± 0.01	0.79 ± 0.39	1.13 ± 0.26
PPAR-γ	BAT	1.01 ± 0.10	1.47 ± 0.30	1.37 ± 0.19	0.86 ± 0.11
	GM	1.57 ± 0.80	0.96 ± 0.29	0.72 ± 0.26	0.56 ± 0.31
Dio2	BAT	1.21 ± 0.49	3.03 ± 0.30***	2.14 ± 0.48	1.25 ± 0.28###
	GM	1.16 ± 0.36	1.41 ± 0.27	0.72 ± 0.07	1.70 ± 0.24

Results are means ± sem. n = 3–5, normalized to HPRT, relative to WT vehicle mice. **P < 0.01 and ***P < 0.001 vs. control; ^##P < 0.01 and ^###P < 0.001 WT AMG910 as determined by 2-tailed Student’s t test.