. Author manuscript; available in PMC: 2015 Nov 18.

Published in final edited form as: Biochemistry. 2009 Feb 10;48(5):929–940. doi: 10.1021/bi802027g

Table 3.

Concentration-Dependent Amplitude Shifts of in Vitro Complementation of t7SPm

	amplitude^a (%)
[s7 peptide] (µM)	A₁	A₂	A₃
14.40	112.10 ± 0.57	−8.07 ± 0.93	−4.02 ± 1.50
7.20	96.48 ± 0.60	21.53 ± 1.47	−18.01 ± 0.86
3.60	70.48 ± 0.08	55.34 ± 0.44	−25.82 ± 0.36
1.80	51.05 ± 3.15	82.56 ± 2.96	−33.61 ± 0.18

In vitro complementation assays of 0.1 µM t7SPm were performed with various concentrations of s7 under native conditions. Time-dependent changes in fluorescence were best fitted to the triple-exponential equation with fixed rate constants derived from the same measurement with 1.8 µM s7 peptide (i.e., k₁ = 0.03810, k₂ = 0.00630, and k₃ = 0.00158). Amplitudes are provided in relative percentage (±SD; n = 2).