Fig. 4.

Deconvoluted mass distributions of (a) WT Im7, (b) DM Im7, and (c) TM Im7. Mass distribution of the H₂O₂ treated (but not laser irradiated) samples are shown in black, and mass distributions of the laser irradiated samples are shown in colour. Peaks corresponding to the protein precursors are labelled 0, with other peaks labelled according to the number of oxygen atoms incorporated as a result of oxidative labelling (1, 2, 3, etc). (d) Estimated % of unmodified protein remaining after FPOP (mean ± SEM of three replicates analysed in triplicate is shown as bars) and estimated % helical content as determined by CD spectroscopy (shown as white squares) [25], [28].