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. 2015 May 13;107(7):djv135. doi: 10.1093/jnci/djv135

Figure 1.

Figure 1.

Effects of neuroblastoma (NBL)–derived exosomic microRNAs (miRNAs) in human monocytes. A) Quantitative real-time polymerase chain reaction (qRT-PCR) for miR-21, miR-29a, and miR-155 in exosomes derived from: 1) SK-N-BE(2), 2) CHLA-255, 3) IMR-32 cells, or 4) Remaining supernatant after exosome isolation (negative control). Exosomes were isolated from NBL cells cultured media containing exosome-free fetal bovine serum for a period of 48 hours. B) qRT-PCR for mature miR-21 in monocytes cocultured with SK-N-BE(2) or CHLA-255 cells for the indicated time periods. C) qRT-PCR for pre-miR-21 in monocytes cocultured with SK-N-BE(2) or CHLA-255 cells for the indicated time periods. D) qRT-PCR for mature miR-21 in monocytes cultured for the indicated time periods with the supernatant (SN) of SK-N-BE(2) cells (normal SN) or with exosome-depleted by ultracentrifugation (depleted SN) of SK-N-BE(2) cells. E) qRT-PCR for mature miR-21 in monocytes treated for the indicated time periods with whole exosomes fraction (WEF) isolated from SK-N-BE(2) cells compared with PBS. F) qRT-PCR for mature miR-21 in monocytes transfected with total RNA extracted from exosomes of SK-N-BE(2) cells (compared with a total scrambled RNA) for the indicated time periods. Relative levels of pre-miRNA or mature miRNAs expression were normalized to GAPDH mRNA or U6 snRNA, respectively. Data are presented as mean ± SD of experiments conducted in triplicate. *P = .004, **P = .002, ***P = .001, ****P < .001, Student’s t test, two-sided.