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Chiral-HPLC analysis of the conversion of DCA-C by SME110 and SME111. DCA-C (200 μM) was incubated with resting cells of SME110 and SME111 (OD600 of 1.0). Portions of the reaction mixtures were collected at the start of incubation (incubation with SME110) (A) and after 5 h of incubation with SME110 (B) and SME111 (C) and analyzed by chiral HPLC.
