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Stereospecificities of PhcC and PhcD for the oxidation of DCA-C enantiomers. (A) Chiral-HPLC profiles of the racemic DCA-C. The membrane fractions containing PhcC (B) or PhcD (C) were incubated with 200 μM DCA-C in the presence of 300 μM FAD and 300 μM PMS for 10 min. The remaining DCA-C in the reaction mixtures was separated by thin-layer chromatography and then analyzed by chiral HPLC.
