TABLE 2.
Enzyme activities of cell extract, soluble fraction, and membrane fraction of SYK-6 toward DCA and its derivatives
| Substrate | Fraction | Cofactor(s) | Activitya (mU) |
||
|---|---|---|---|---|---|
| SEMP | SEMP + DCA | SEMP + DCA-C | |||
| DCA | Cell extract | None | 21 ± 7 | 36 ± 3 | 46 ± 10 |
| NAD+ | 160 ± 2 | 170 ± 2 | 140 ± 1 | ||
| PQQ + PMS | 61 ± 2 | 59 ± 0.6 | 56 ± 0.2 | ||
| FAD + PMS | 43 ± 0.8 | 73 ± 3 | 74 ± 4 | ||
| Soluble | None | 5.7 ± 3 | 11 ± 6 | —c | |
| NAD+ | 110 ± 2 | 120 ± 2 | — | ||
| PQQ + PMS | 20 ± 0.4 | 16 ± 2 | — | ||
| FAD + PMS | 15 ± 3 | 16 ± 1 | — | ||
| Membrane | None | 6.8 ± 0.4 | 12 ± 0.4 | — | |
| NAD+ | 6.3 ± 0.1 | 13 ± 0.7 | — | ||
| PQQ + PMS | 18 ± 0.2 | 18 ± 0.1 | — | ||
| FAD + PMS | 9.4 ± 0.3 | 21 ± 0.8 | — | ||
| DCA-C | Cell extract | None | 3.5 ± 0.4 | 14 ± 0.8 | 6.0 ± 0.6 |
| NAD+ | 2.4 ± 1 | 14 ± 0.2 | 6.4 ± 1 | ||
| PQQ + PMS | 3.2 ± 0.5 | 16 ± 0.3 | 9.1 ± 2 | ||
| FAD + PMS | 19 ± 0.6 | 32 ± 0.4 | 27 ± 0.7 | ||
| Soluble | None | NDb | 1.7 ± 0.2 | — | |
| NAD+ | ND | 1.4 ± 0.5 | — | ||
| PQQ + PMS | 1.3 ± 0.3 | 3.0 ± 0.1 | — | ||
| FAD + PMS | 5.3 ± 0.6 | 6.9 ± 0.2 | — | ||
| Membrane | None | 0.8 ± 0.04 | 3.0 ± 0.1 | — | |
| NAD+ | 0.7 ± 0.05 | 3.1 ± 0.08 | — | ||
| PQQ + PMS | 0.8 ± 0.1 | 3.2 ± 0.05 | — | ||
| FAD + PMS | 2.4 ± 0.02 | 3.8 ± 0.07 | — | ||
| DCA-CC | Cell extract | None | 1,300 ± 30 | 2,400 ± 70 | 2,200 ± 100 |
| Soluble | None | 1,300 ± 20 | 2,100 ± 10 | — | |
| Membrane | None | ND | ND | — | |
| DCA-S | Cell extract | None | 180 ± 40 | 240 ± 20 | — |
| Soluble | None | 130 ± 8 | 190 ± 20 | — | |
| Membrane | None | ND | 4.1 ± 2 | — | |
a
Cell extracts of SYK-6 (10 mg of protein) were fractionated into the soluble (7.5 mg of protein) and membrane (0.94 mg of protein) fractions. Enzyme activities in the cell extract, soluble fraction, and membrane fraction are shown as milliunits/10 mg of protein, milliunits/7.5 mg protein, and milliunits/0.94 mg of protein, respectively. The data are averages ± standard deviations from three independent experiments.
b
ND, not detected.
c
—, not tested.