TABLE 4.

Type VI secretion system in Helicobacter pullorum strains isolated from chicken meat^a

Label	Locus tag for strain:			Predicted protein/putative domains	BLASTp against C. jejuni 414b
	MIT 98-5489	229254/12	229336/12		Cover (%)	Identity (%)	E value
A	HPMG_00002	HPU229254_00020	HPU229336_00010	Flagellar hook-associated protein (FlgK)
B	HPMG_00003	HPU229254_00030	HPU229336_00020	Predicted T6SS component/FHA domain	98	52	3e−93
C	HPMG_00004	HPU229254_00035	HPU229336_00025	T6S protein IcmF/VasK	64	64	0
D	HPMG_00005	HPU229254_00040	HPU229336_00030	T6S effector Hcp1 family/T6SS component Hcp (secreted cytotoxin)	100	82	8e−111
E	HPMG_00006	HPU229254_00045	—c	Acetyltransferase (GNAT) family
F	HPMG_00007	HPU229254_00050	HPU229336_00045	Helicase/NYN domain
G	HPMG_00008	HPU229254_00055		Helicase
H	HPMG_00009	HPU229254_00060	HPU229336_00055	Hypothetical protein
I	HPMG_00010	HPU229254_00070	HPU229336_00060	T6S protein ImpH/VasB	100	61	6e−132
J	HPMG_00011	HPU229254_00075	HPU229336_00065	T6S protein ImpG/VasA	100	63	0
K	HPMG_00012	HPU229254_00080	HPU229336_00070	T6SS lysozyme-like protein	98	61	1e−23
L	HPMG_00013	HPU229254_00085	HPU229336_00075	T6S protein ImpC	100	83	0
M	HPMG_00014	HPU229254_00090	HPU229336_00080	T6S protein ImpB	83	77	7e−72
N	HPMG_00015d	HPU229254_00095	HPU229336_00085	T6S ImpA-related N-terminal protein	74	41	5e−72
O	HPMG_00016	HPU229254_00100	HPU229336_00090	T6S lipoprotein VasD/SciN	80	69	2e−58
P	HPMG_00017	HPU229254_00105	HPU229336_00095	T6S protein ImpJ/VasE	99	67	0
Q	HPMG_00018	HPU229254_00110	HPU229336_00100	T6S protein ImpK/VasF; OmpA/MotB domain	81	62	6e−84
R	HPMG_00019	HPU229254_00115	HPU229336_00105	Hypothetical protein/restriction endonuclease-like superfamily
S	HPMG_00020	—e	HPU229336_00110	Hypothetical protein/phosphatidylinositol-4-phosphate 5-kinase
T	HPMG_00021	—e	HPU229336_00115	Hypothetical protein
U	HPMG_00022			Hypothetical protein
V	HPMG_00023			Hypothetical protein
W	HPMG_00024	HPU229254_00125	HPU229336_00120	T6SS VgrG protein/phage late control gene D protein	92	47	0
X	HPMG_00025	HPU229254_00130	HPU229336_00125	Hypothetical protein
Y	HPMG_00026	HPU229254_00135	HPU229336_00130	Hypothetical protein/predicted ATP-dependent endonuclease of the OLD family
Z	HPMG_00027	HPU229254_00140	HPU229336_00135	Hypothetical protein

^aT6SS genes are highlighted in bold.

^bBLASTp result (nonredundant protein sequence, blastp) of the T6SS proteins from Helicobacter pullorum against the genome of Campylobacter jejuni subsp. jejuni 414 (GenBank accession number NZ_CM000855) described in reference 41.

^cThe 229336/12 strain has two putative ORFs (HPU229336_00035 and HPU229336_00040) in this region.

^dThis region has annotation issues in the genome of the strain MIT 98-5489.

^eThe 229254/12 strain has a putative gene fusion (HPU229254_00120) encompassing the ORFs HPMG_00020 and HPMG_00021.