TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| M. gryphiswaldense | ||
| MSR-1 | WT; Nxr | DSM 6361 |
| ΔirrB mutant | irrB-defective mutant; Nxr Gmr | This study |
| CF3149 | Complemented strain of the ΔirrB mutant; Nxr Gmr Tcr | This study |
| Δfur mutant | fur-defective mutant; Nxr Gmr | 11 |
| E. coli | ||
| DH5α | endA1 hsdR17(rK− mK+) supE44 thi-1 recA1 gyrA (Nalr) recA1 Δ(lacZYA-argF)U169 deoR[ϕ80dlacZΔM15] | 41 |
| S17-1 | thi endA recA hsdR with RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome; Smr | 42 |
| Plasmids | ||
| pMD 18 T-simple | Cloning vector; Ampr | TaKaRa |
| pUCGm | pUC1918 carrying the aacC1 gene, Gmr | 43 |
| pUX19 | Suicide vector for M. gryphiswaldense MSR-1; Kmr | 44 |
| pUXF | pUX19 containing Gm cassette, irrB upstream and downstream regions; Kmr Gmr | This study |
| pRK415 | Broad-host-range cloning vector; Tcr | 45 |
| pRK415F | pRK415 containing irrB from M. gryphiswaldense MSR-1 | This study |