TABLE 3.

Kinetic constants for short-chain dehydrogenases from SG61-1L and SYK-6 on all GGE stereoisomers

Constant and name	(αS,βR)-GGE	(αR,βS)-GGE	(αR,βR)-GGE	(αS,βS)-GGE
Km, GGE (μM)
LigD	0.39 ± 0.08	15 ± 13	25 ± 10	12.2 ± 2.3
LigL	1.6 ± 0.3	39 ± 5	3.0 ± 0.5	61 ± 16
LigN	51 ± 14	15.3 ± 2.8	1.7 ± 0.3	35.0 ± 7.2
LigO	118 ± 29	1.2 ± 0.4	51 ± 12	40.3 ± 9.6
2550	3.6 ± 0.6	53 ± 9	2.2 ± 0.8	0.8 ± 0.2
1498	88 ± 15	NDa	ND	57 ± 12
2705	357 ± 47	235 ± 41	ND	354 ± 44
3329	ND	ND	49 ± 16	35 ± 10
3344	66 ± 12	202 ± 16	19.0 ± 3.0	162 ± 24
474	109 ± 18	ND	ND	163 ± 22
724	77 ± 18	2.0 ± 0.4	4.2 ± 0.8	33.7 ± 8.8
kcat (s−1)
LigD	4.9 ± 0.2	17.0 ± 3.7	22.7 ± 2.9	8.4 ± 0.4
LigL	83.4 ± 0.1	76.5 ± 3.8	33.9 ± 1.0	381 ± 0.6
LigN	50.6 ± 5.1	65.2 ± 3.1	25.4 ± 1.0	67.4 ± 5.6
LigO	7.0 ± 0.7	2.8 ± 0.2	3.0 ± 0.3	15.6 ± 1.5
2550	46.1 ± 2.3	117 ± 6	15.9 ± 1.5	74.2 ± 2.7
1498	3.9 ± 0.3	ND	ND	2.6 ± 0.2
2705	50.8 ± 3.0	2.8 ± 0.2	ND	66.1 ± 4.0
3329	ND	ND	0.6 ± 0.1	0.1 ± 0.01
3344	2.3 ± 0.2	2.3 ± 0.1	0.3 ± 0.01	197 ± 10
474	1.5 ± 0.1	ND	ND	12.5 ± 0.8
724	942 ± 83	701 ± 27	1534 ± 62	722 ± 66
kcat/Km, GGE (μM−1 s−1)
LigD	12.5 ± 2.7	1.14 ± 1.06	0.92 ± 0.4	0.68 ± 0.13
LigL	53.3 ± 9.0	1.9 ± 0.3	11.3 ± 2.1	6.3 ± 1.7
LigN	1.0 ± 0.3	4.2 ± 0.8	15.1 ± 2.9	2.0 ± 0.4
LigO	0.06 ± 0.01	2.4 ± 0.8	0.06 ± 0.01	0.39 ± 0.10
2550	12.9 ± 2.4	2.2 ± 0.4	7.4 ± 2.7	95.1 ± 20
1498	0.04 ± 0.01	ND	ND	0.05 ± 0.01
2705	0.14 ± 0.02	0.012 ± 0.002	ND	0.18 ± 0.03
3329	ND	ND	0.012 ± 0.004	0.003 ± 0.001
3344	0.03 ± 0.01	0.011 ± 0.001	0.015 ± 0.003	1.2 ± 0.2
474	0.013 ± 0.002	ND	ND	0.08 ± 0.01
724	12.4 ± 3.1	360 ± 74	368 ± 69	21.4 ± 6.0

^aND, no detectable activity.