TABLE 2.
PHA accumulation in recombinant E. coli LS5218 harboring parental or Val72/Ile72 mutant genes of phaJ1a
| Plasmids (relevant marker[s]) | Dry cell wt (g/liter) | PHA content (%, wt/wt) | PHA compositionb (mol%) |
||||
|---|---|---|---|---|---|---|---|
| 3HB (C4) | 3HHx (C6) | 3HO (C8) | 3HD (C10) | 3HDD (C12) | |||
| pPPAc, pUC19c (phaC1Ps) | 0.39 ± 0.09 | 4 ± 2 | 1 | 21 | 49 | 19 | 10 |
| pPPAC, pUCJ1Ppc (phaC1Ps, phaJ1Pp) | 0.54 ± 0.08 | 19 ± 2 | 9 | 36 | 37 | 11 | 7 |
| pPPAC, pUCJ1PpV72A (phaC1Ps, phaJ1Pp V72A) | 0.66 ± 0.09 | 7 ± 1 | 5 | 21 | 42 | 19 | 13 |
| pPPAC, pUCJ1PpV72I (phaC1Ps, phaJ1Pp V72I) | 0.58 ± 0.04 | 31 ± 3 | 12 | 67 | 14 | 4 | 3 |
| pPPAC, pUCJ1PpV72L (phaC1Ps, phaJ1Pp V72L) | 0.74 ± 0.02 | 30 ± 2 | 12 | 69 | 10 | 5 | 4 |
| pPPAC, pUCJ1 (phaC1Ps, phaJ1Pa) | 0.73 ± 0.02 | 32 ± 2 | 13 | 74 | 8 | 3 | 2 |
| pPPAC, pUCJ1Pa I72V (phaC1Ps, phaJ1Pa I72V) | 0.58 ± 0.16 | 32 ± 2 | 13 | 60 | 20 | 4 | 3 |
a
Cells harboring PHA synthase 1 gene (phaC1Ps) from Pseudomonas sp. strain 61-3 were cultivated for 96 h at 37°C in M9 medium containing sodium dodecanoate (0.25%, wt/vol) as the sole carbon source. The surfactant Brij-35 (0.4%, wt/vol) was added to each medium to solubilize dodecanoate. All values are averages and standard deviations from triplicate tests.
b
Abbreviations: 3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate.
c
Data were retrieved from reference 10.