TABLE 4.
PHA accumulation in recombinant E. coli LS5218 harboring chimeric phaJ1 genesa
| Plasmidsb (relevant markers) | Dry cell wt (g/liters) | PHA content (%, wt/wt) | PHA compositionc (mol%) |
||||
|---|---|---|---|---|---|---|---|
| 3HB (C4) | 3HHx (C6) | 3HO (C8) | 3HD (C10) | 3HDD (C12) | |||
| pPPAC, pUCJ1ch67 (phaC1Ps, phaJ1ch67) | 0.54 ± 0.11 | 18 ± 4 | 14 | 50 | 30 | 4 | 2 |
| pPPAC, pUCJ1ch172 (phaC1Ps, phaJ1ch172) | 0.51 ± 0.09 | 22 ± 3 | 14 | 50 | 32 | 3 | 1 |
| pPPAC, pUCJ1ch372 (phaC1Ps, phaJ1ch372) | 0.80 ± 0.05 | 30 ± 3 | 14 | 75 | 8 | 2 | 1 |
a
Cells harboring PHA synthase 1 gene (phaC1Ps) from Pseudomonas sp. strain 61-3 were cultivated for 96 h at 37°C in M9 medium containing sodium dodecanoate (0.25%, wt/vol) as the sole carbon source. The surfactant Brij-35 (0.4%, wt/vol) was added to each medium to solubilize dodecanoate. All values are averages and standard deviations from triplicate tests.
b
phaJ1ch67, phaJ1ch172, and phaJ1ch372 were chimeric (R)-hydratase genes (see footnote b to Table 3).
c
Abbreviations: 3HB, 3-hydroxybutyrate; 3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate; 3HDD, 3-hydroxydodecanoate.