Figure 3.

Daptomycin dissipates the membrane potential in B anthracis, as measured by DiSC₃(5). CON, DAP, PEN, CIP, NISIN, and CCCP represent the control, daptomycin, penicillin G, ciprofloxacin, nisin, and CCCP, respectively. The fluorescence intensity is expressed as the mean signal of the 5-min trace. An increase in fluorescence indicates membrane depolarization. When exposed to daptomycin for 30 min, the membrane potential is dissipated in vegetative B anthracis (A) and germinating spores (B). The data are representative of those from three independent experiments. ^cP<0.01.