Figure 4.

Daptomycin dissipates the membrane potential in B anthracis, as measured by flow cytometry. CON, DAP, PEN, CIP, NISIN, and CCCP represent the control, daptomycin, penicillin G, ciprofloxacin, nisin, and CCCP, respectively. The fluorescence ratio (red/green) represents the membrane potential. When the fluorescence ratio decreased (30 min), the membrane potential is reduced. The results of antibiotics against vegetative B anthracis (A) and spores (B) are consistent with those by DiSC₃(5), as above. The data are representative of those from three independent experiments. ^bP<0.05, ^cP<0.01.