Skip to main content
. 2013 Dec 30;35(2):257–266. doi: 10.1038/aps.2013.165

Figure 1.

Figure 1

Mangiferin (MA) increased accumulation of Nrf2 protein in HL-60 cells. (A) HL-60 cells were incubated with 50 μmol/L mangiferin for 15 min, 1, 4, or 24 h. Subcellular localization of Nrf2 protein was determined by confocal microscopy using FITC-conjugated Nrf2 antibody (green color). Nuclei were visualized by Hoechst staining (blue color). Merging Nrf2 and nuclei images (cyan color) confirmed nuclear localization of Nrf2 (400×magnification). (B) Cells were treated with 50, 100 or 200 μmol/L mangiferin for 24 h in dose-response studies or (C) incubated with 50 μmol/L MA for 0, 1, 4, 12, or 24 h in time-response studies. Total cell lysates were subjected to western blotting with Nrf2 antibodies. β-Actin was examined as the control for equal protein loading and protein integrity. (D1) Cells were treated with 50 μmol/L MA for 4 h. Subcellular expression of Nrf2 was determined by western blotting. β-Actin or lamin was examined as the control for equal protein loading and protein integrity. (D2) Nrf2 expression determined by western blotting was quantified by densitometry. Data represented the mean±SD of at least three independent experiments (bP<0.05). FITC, fluorescein isothiocyanate. Nrf2, NF-E2-related nuclear factors 2.