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. 2013 Dec 30;35(2):257–266. doi: 10.1038/aps.2013.165

Figure 4.

Figure 4

Mangiferin (MA) relieved oxidative stress in HL-60 cells. HL-60 cells were pre-incubated with 50 μmol/L mangiferin for 4 h and then treated with 20 μg/mL etoposide for 8 h. Cells were loaded with 20 μmol/L of fluorescence probe for ROS (DCFH-DA) for 20 min at 37 °C. Pictures were captured using a fluorescence microscope (400×magnification). IOD of each sample was detected by Image Pro Plus for statistics analysis (bP<0.05 vs control. eP<0.05 vs etoposide treatment alone). Data represented the mean±SD of at least three independent experiments. ROS, reactive oxygen species. DCFH-DA, 2′,7′-Dichlorodihydrofluorescein diacetate. IOD, internal optical density.