Skip to main content
. 2013 Dec 30;35(2):219–229. doi: 10.1038/aps.2013.167

Figure 5.

Figure 5

DZ2002 altered the cytokine-secreting pattern of BMDCs to suppress Th17 cell differentiation in vitro. (A) BMDCs from normal female C57BL/6 mice were treated without (w/o) or with TLR agonists in the presence or absence of DZ2002 (100 μmol/L) for 24 h. Cytokine productions were measured by ELISA. (B) Naïve CD4+ T cells stimulated with anti-CD3 mAb were co-cultured with BMDCs, which were primed without (w/o) and with TLR agonists in the presence (DZ-DC) or absence (DC) of DZ2002 (100 μmol/L), for 4 d. Cytokine productions were measured from the co-culture supernatants by ELISA. (C) Co-cultured T cells were analyzed by flow cytometer. Left: Intracellular staining of IL-17 and IFN-γ in cultured CD4+ T cells. Middle: The percentage of FoxP3+ Tregs in cultured CD4+ T cells was measured. Data shown represent results of 3 independent experiments. Right: Statistical analysis of 3 independent experiments. Mean±SEM. n=3 independent experiments. bP<0.05, cP<0.01 vs corresponding medium with similar stimulatory condition. fP<0.01 vs the culture contained BMDCs without TLR agonist prime.