Fig. 3.

MIR482q evolved from a gene encoding a NB-LRR protein. (A) Stem-loop structures for selected copies of MIR482/MIR2118 or putative intermediate MIRNA precursors. miRNA sequences are highlighted by cyan lines, and miRNA/miRNA* duplexes are enlarged with the size of middle loop(s) represented by yellow circles. Stem-loops bearing the same miRNA sequence are indicated with different background colors. (B) The 3′-arm of MIR482q encodes an appreciable peptide. The position of the mature miRNA (its reverse complement) and the translated amino acid sequence are marked with cyan lines. The one nucleotide frame shift allowing for accurate translation is indicated. (C) Distribution of e values from BLASTP (see Materials and Methods) using the peptide encoded by the sequence of the 3′-arm of MIR482q, searching with no mask (left) or masked conditions (right).