Table 1.
Primer sequences used for RT-PCR and Real-time PCR
| Primer Name | Sequences ( 3′→5′) | Refrence | |
|---|---|---|---|
| Collagen type I ( | COL2A1) Forward | CGTCCAGATGACCTTCCTACG | NM_001844 |
| Colla Collagen type I ( COL2A1) Reverse | TGAGCAGGGCCTTCTTGAG | ||
| Collagen type II (COL1A2) Forward | CAGGAAACAGCTATGACC | NM_000089 | |
| Collagen type II (COL1A2) Reverse | CTACTCTCAGCCCAGGAGGTCCTG | ||
| Aggrecan (ACAN) Forward | AGGCAGCGTGATCCTTACC | NM_001135 | |
| Aggrecan (ACAN) Reverse | GGCCTCTCCAGTCTCATTCTC | ||
| SOX9 Forward | GTACCCGCACTTGCACAAC | NM_000346 | |
| SOX9 Reverse | TCTCGCTCTCGTTCAGAAGTC | ||
| GAPDH Forward | CGCTCTCTGCTCCTCCTGTT | NM_001256799 | |
| GAPDH Reverse | CCATGGTGTCTGAGCGATGT | ||
| β-Actin Forward | AATCTGGCACCACACCTTCTAC | NM_001101 | |
| β-Actin Reverse | CATCTTCCACACCACGGTCTAA | ||