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. 2015 Nov 18;10(11):e0140826. doi: 10.1371/journal.pone.0140826

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 1 — CLas copy number in D. citri samples (three replicates) was estimated using qPCR targeting the 16s rRNA gene. Ct values from biological samples were compared to a dilution series of a synthetic plasmid corresponding to the CLas 16s rRNA target gene. Natural logarithm of estimated CLas copy number per insect equivalent is shown on the Y-axis. All reactions were performed in triplicate (mean plus standard deviation). No signal was observed within 40 cycles for CLas(-) samples.