Table 6. Verification of gene expression changes by qRT-PCR.
| Gene symbol | Expression in sterile gonads | Sequence of primers used in q-PCR | Expression of fold change (log2) | |
|---|---|---|---|---|
| RNA-seq | qPCR | |||
| TGFB3 | over | CAAGATTGGTCTGTCGGTA (F) | -1.25 | -2.34 |
| AGCCAAACAGCGTTACAT (R) | ||||
| CYP11A1 | under | ACGCCTGTTTGACCTCTG (F) | 4.69 | 3.81 |
| TGAGCATTTCTGTTGGGAG (R) | ||||
| CPA1 | over | GGTGTAAGCGTAGCCGTCAG (F) | -4 | -3.85 |
| TCCAGGGAATGGGTGTCG (R) | ||||
| CTSS | over | CAAACAAGCGAGTTATGAT (F) | -1.36 | -1.6 |
| AACCTCTTTCGGAGACAA (R) | ||||
| BCL2L1 | under | GTGATGGACGAGGTGTTC (F) | 1.22 | 0.19 |
| ATCCTGTCCACCAGCGAA (R) | ||||
| ABCA1 | over | GCCACAGAGTGCCGTTAT (F) | -2 | -3.1 |
| TTCCTTCGGGTGATGAGT (R) | ||||
| TNFSF10 | over | CCTCGGTTCAAAGATGGAT (F) | -1.22 | -2.18 |
| CGTTGGCAAAGCAAGGAA (R) | ||||
| HSD3β | under | TCGTATCCCGTCATCCAT (F) | 1.84 | 0.73 |
| TGAGGTTTTCCTACAGCAAG (R) | ||||
| CYP11B2 | under | TATTCTGCGTCCCAAACA (F) | 4.67 | 3.78 |
| TTCGTCCAGAGCAGTATCA (R) | ||||
| PRLR | under | CAATAATGAGCCAATAACG (F) | 5.12 | 4.06 |
| GTGGGTCTGTGGATGTTA (R) | ||||