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Total RNA was extracted from tissues, integrity evaluated (all the samples included showed a RIN above 9) and changes in expression of SIAH2 in tumor samples compared to normal lung samples from the same patient analyzed by qPCR and expressed as a fold-change. Amplification efficiencies were validated and normalized against β-actin and HPRT, and fold change in gene expression was calculated using the 2−ΔΔCt method. Results represent the mean ± SD.
