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. 2015 Nov 18;10(11):e0143077. doi: 10.1371/journal.pone.0143077

Fig 8. Effect of AgNP on the internalization of M.tb by MDM.

Fig 8

MDM were infected with M.tb at MOI 10 in the absence or presence of 10 μg/mL of AgNP and the percentage of MDM with internalized M.tb determined after 2 (panel a) and 4 (panel b) hours of infection. A total of 300 MDM was examined in each experimental condition as described in Materials and Methods. Results are expressed as mean percentages of MDM with internalized M.tb ± standard deviations from four independent experiments. To assess the number of internalized M.tb in MDM, MDM infected with M.tb (MOI 1) alone, MDM infected with M.tb and exposed to Ag20-citrate (10 μg/mL) and MDM infected with M.tb and exposed to Ag110-citrate (10 μg/mL) were lysed following a 4-hour incubation. MDM lysates were then plated onto agar plates and CFU determined as described in Materials and Methods. Panel c shows the mean number of CFU ± standard deviation from three independent experiments.