Fig 2. Edar expression and NF-κB reporter expression co-localize in the mammary epithelium.

(A) Edar transcripts were detected by in situ hybridization with a ³⁵S-UTP-labeled probe in mammary buds at E12.5 and E13.5. (B) NF-κB reporter was initially expressed throughout the mammary bud in control embryos, but became localized to the basal layer of the epithelium around E13.5. (C) In K14-Eda embryos, reporter activity stayed high throughout the mammary bud at E12.5 and E13.5. mb4 = mammary bud number 4. (D) The supernumerary buds exhibited mosaic expression of the reporter which was less pronounced in the in the neck (left) than in mammary primordia forming between buds 3 and 4 (right). (Scale bar: 100 μm.)