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. Author manuscript; available in PMC: 2016 Apr 23.
Published in final edited form as: Cell. 2015 Apr 23;161(3):581–594. doi: 10.1016/j.cell.2015.03.048

Figure 4.

Figure 4

Nanoclustering of GPI-AP at the Cell Surface Requires Long-Acyl-Chain-Containing PS (A–D) Cropped fluorescence intensity and anisotropy images (A and C) and cumulative frequency distributions (B and D) of A488F-labeled PSA3 mutant CHO cells grown with (PS replete [PS+; red line] or without [PS deplete (PS_); green line]) ethanolamine and supplemented with PLA2-insensitive PS analogs of indicated acyl chain lengths and saturation (A and B; blue or black line) or supplemented with PLA2 inhibitor and PLA2-sensitive PS analogs of indicated acyl chain saturation (C and D; blue and black lines). (E) Chemical structure depicts the PLA2-insensitive (black) and -sensitive (gray) PS analogs used in (A)–(D) above, respectively.

See also Figures S4 and S5.