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. Author manuscript; available in PMC: 2016 Jul 29.
Published in final edited form as: Cell Syst. 2015 Mar 3;1(1):72–87. doi: 10.1016/j.cels.2015.01.001

Figure 1. The Metagenome of New York City.

Figure 1

(A) The five boroughs of NYC include (1) Manhattan (green), (2) Brooklyn (yellow), (3) Queens (orange), (4) Bronx (red), (5) Staten Island (lavender).

(B) The collection from the 466 subway stations of NYC across the 24 subway lines involved three main steps: (1) collection with Copan Elution swabs, (2) data entry into the database, and (3) uploading of the data. An image is shown of the current collection database, taken from http://pathomap.giscloud.com.

(C) Workflow for sample DNA extraction, library preparation, sequencing, quality trimming of the FASTQ files, and alignment with MegaBLAST and MetaPhlAn to discern taxa present.

(D) Distribution of taxa identified from the entire pooled dataset.

(E) Geospatial analysis of the most prevalent genus, Pseudomonas, across the subway system; hotspots reveal high density of Pseudomonas in areas in Manhattan and Brooklyn.