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. 2015 Nov 18;10(11):e0142809. doi: 10.1371/journal.pone.0142809

Fig 3. Fat droplet detection after transecting with miR-130b mimic or inhibitor in GMEC.

Fig 3

Changes in the lipid contents of GMECs transfected with miR-130b mimic (60nM) or miR-130b inhibitor (60nM). Cells were stained by Oil Red. After examined microscopically, the oil red O was extracted with 400 μl of isopropanol and its absorbance was determined at 510 nm. The relative fat droplet content was normalized to control transfected cells. A: NC mimic treatment, B: miR-130b mimic treatment, C: NC inhibior treatment, D: miR-130b inhibior treatment. E: fat droplet content in cells. Fat droplet was compared with that of control (n = 6). White bars, negative control; black bars, miR-130b mimic or inhibitor. All experiments were performed in duplicate and repeated three times. Values are presented as means ± standard errors, *, P<0.05; **, P<0.01.