Fig 4. Akt inhibition by luteolin is involved in its effect on ceramide metabolism.

(A) CCs were treated with 50 and 100 μM of luteolin for 2 h and submitted to immunoblotting with anti-pAKT antibodies. β-actin was used as loading control. (B) Cells were submitted to pulse with ³H-Sph in the absence (CT) or presence of LY294002 for 2h. The levels of ceramide (Cer), sphingomyelin (SM) and glycosphingolipids (GSLs) are reported as mean ± S.D. of at least three independent experiments. **, p < 0.01 vs CT cells.