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. 2015 Nov 18;10(11):e0143106. doi: 10.1371/journal.pone.0143106

Table 1. Primers used in this study a .

Primer set Primer name Sequence (5'-3') Reference
Detection of RI integrated into groEL in S. epidermidis
a S. epi groEL 1213-1232F CTKGAAGAAGGTATYGTTGC [15]
int (I) 109-128F CGTAAATCAGACGCTAAACA [15]
b int (I) 109-128F CGTAAATCAGACGCTAAACA [15]
int (I) 1139-1122R CTAAACTTGTGGGAAGCG [15]
c fusB531-559F CGGATGGTCAATATGTAAAAAAAGGTGAC [13]
185 LA 3R CTCACAGAGGTTCTATAATGTTGG [15]
Detection of RI integrated into smpB in S. epidermidis
d S.epi ssra407-429 (F) TCAAGCACTTAAAGAAAAAGCGG [16]
int (III) 175–194F GACGAGTTAGAGGGTATTGG [16]
e int (III) 175–194F GACGAGTTAGAGGGTATTGG [16]
int (III) 1087–1068R TACTAGGGTACAAATGACCG [16]
f fusB531-559F CGGATGGTCAATATGTAAAAAAAGGTGAC [13]
S.epi sodium transporter 1146–1168F TCTCACTATGGATTTAACTTCCG [16]
Detection of RI integrated into rpsR in S. epidermidis
g S epi rpsR 6-24F AGGTGGACCAAGAAGAGGC [15]
int(II) 541-565F GCTAAACGTAATAACTATTTAGAAG [15]
h int(II) 541-565F GCTAAACGTAATAACTATTTAGAAG [15]
int 1061-1042R GTGTGACGTAATGTGTGCGT [15]
i fusB531-559F CGGATGGTCAATATGTAAAAAAAGGTGAC [13]
fusB LA-2R AATACTCCTGGATGGCGT [15]
Detection of RI integrated into groEL in S. capatis subsp. urealyticus (primer sets a and b also used)
j fusB531-559F CGGATGGTCAATATGTAAAAAAAGGTGAC [13]
cap-CWA-R CYTCMTCTTCGTCAGGAT This study
Sequencing of ScRIfusB (primer sets a, b, and j also used)
k 7778 PstI up2F CGCTGATACCTTTGTTGAAC This study
fusBR ACAATGAATGCTATCTCGACA [13]
j Sepi 2793up2F AAAGTGCTGTATGGCGTG This study
ri17 284-265R TCCATAGCATTTAATCCGTG This study
Detection of the fusB-carrying plasmid pUB101 and its relatives
m IS257 518-499R ATATGACGGTGATCTTGCTC [13]
fusB 283-254R AGGTAGTTCAAAAG [13]
n IS257 33-52F GGATGTTATCACTGT [13]
fusB 530-558F CGGATGGTCAATATGTAAAAAAAGGTGAC [13]
Detection and sequencing SCCfusC
o orfX-uF ACTTCGTCTTCGTCATTGG This study
hsdR_593R CTCCAATAAAACATTTGTCCC [17]
p (inverse PCR) SAS0044dn382R GGATTCAGAATGGTTTCC [17]
SAS0046_226R AACCTTCGGTATCATCCG This study
Sequencing of pseudo SCC and its flanking region
q (LA PCR) fusC 162-183F GGACTTTATTACATCGATTGAC [13]
fusC 572-550R CTGTCATAACAAATGTAATCTCC [13]
r (inverse PCR) 21429-helicase-R CGGCTTGAAACTGTAACC This study
21429A-copBR GTATGACAAGTATCGCAGCG This study
s (inverse PCR) copB-F ATACGAGTTGGTGAAACCTTAC This study
TFGfusC 7914F TAACGGTCATTTCACTCG This study
t MFS-transF GAACAGATTTAGCAAAGTCAC This study
speG-7F CTAAGAGCATTAGAGTATAGTG [17]
u (inverse PCR) up speG-R GATTTGTATGAATGGCACTC This study
speG 408R TGTTTTAAATCCTTGTGACTCG [17]
v speGR408R TGTTTTAAATCCTTGTGACTCG [17]
hominis-afSCC-R TTCTTCTGAAACTATCTGCTGG This study

a The positions of the primers are indicated in S1 Fig (fusB-carrying elements) or S2 Fig (fusC-carrying elements).