Table A1.

PCR mastermix reagents.

Reagent	Volume (μL)	Final concentration
Nuclease-free water	30.5
5× Phusion HF	10	1×
10 mM dNTPs	1	200 μM
10 μM Forward primer	2.5	0.5 μM
10 μM Reverse primer	2.5	0.5 μM
2 U/μL Phusion DNA polymerase	0.5	0.4 U/20 μL PCR
Template DNA	3	150 ng (total amount)
Total	50