Table A3.
PCR conditions for ITS2 locus.
| Step | Cycles | Temperature (°C) | Time |
| Initial denaturation | 1 | 98 | 30 s |
| Denaturation | 30 | 98 | 10 s |
| Annealing | 30 | 59 | 30 s |
| Extension | 30 | 72 | 30 s |
| Final extension | 1 | 72 | 10 min |
| Hold | 1 | 4 | ∞ |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.