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. 2015 Oct 30;3(11):apps.1500043. doi: 10.3732/apps.1500043

Table A5.

PCR conditions for rbcL locus.
Step Cycles Temperature (°C) Time
Initial denaturation 1 98 30 s
Denaturation 30 98 10 s
Annealing 30 57 30 s
Extension 30 72 30 s
Final extension 1 72 10 min
Hold 1 4