| Blunt/TA Ligase Master Mix (red) | 15 μL |
| NEBNext Adaptor for Illumina* (red) | 2.5 μL |
| Ligation Enhancer (red) | 1 μL |
| Total volume | 83.5 μL |
*The NEBNext Adaptor is provided in the Multiplex Oligos for Illumina kit.
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