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. 2015 Nov 5;2015:630287. doi: 10.1155/2015/630287

Figure 1.

Figure 1

Generation of recombinant mouse AMHR2-CD. (a) Schematic representation of full-length AMHR2 showing the extracellular, transmembrane, and cytoplasmic domains with a C-terminal 6xHis-tagged AMHR2-CD variant. (b) Expression of AMHR2-CD in noninduced, IPTG-induced, and Ni-NTA affinity purified AMHR2-CD shown on an SDS-PAGE gel stained with Coomassie blue. (c) Anti-His Western blot of SDS-PAGE gel showing expression of AMHR2-CD in noninduced, IPTG-induced, and two doses of Ni-NTA affinity purified AMHR2-CD.