Figure 5.

Tumor analysis. (a) Arrows show extensive infiltration of CD3+ T cells in an ID8 tumor from AMHR2-CD vaccinated mice in lower resolution (upper panel) and higher resolution (lower panel) images. Inflammatory infiltrates of CD3+ T cells were never observed in control mice vaccinated with CFA alone. (b) Flow cytometry analysis of TILs gated on the CD3+ T cell population showed a pronounced increase in percentages of CD4+ T cells but not CD8+ T cells in tumor infiltrates from mice vaccinated with AMHR2-CD compared to control mice immunized with CFA alone. Data shown are representative of three experiments yielding similar results. (c) Tumors from AMHR2-CD immunized mice consistently showed increased relative gene expression for CD4, IFNγ, TNFα, NKR-P1A, and IL-2 but not for CD8. Asterisks indicate statistical significance. Error bars show ±SD.