Table 1.
Primer pairs used for cloning, qRT-PCR, detection of transgene, and conventional RT-PCR.
| Protein | Sequence (5′-3′) | Amplicon length (bp) |
|---|---|---|
| Cloning of AMHR2-CD | ||
| AMHR2-CD | ||
| Forward | GGATCCAAGGCCTGCAGAGTGCAAGGTG | 1209 |
| Reverse | AAGCTTCTACTCATTTACATACACCTG | |
| TgMISIIR-TAg transgene expression | ||
| SV40-TAg | ||
| Forward | TGCATGGTGTACAACATTCC | 773 |
| Reverse | TTGGGACTGTGAATCAATGCC | |
| qRT-PCR | ||
| IFNγ | ||
| Forward | GGATATCTGGAGGAACTGGCAA | 110 |
| Reverse | TGATGGCCTGATTGTCTTTCAA | |
| TNFα | ||
| Forward | CGAGTGACAAGCCTGTAGCC | 209 |
| Reverse | GTGGGTGAGGAGCACGTAGT | |
| IL-2 | ||
| Forward | GCAGGCCACAGAATTGAAAG | 207 |
| Reverse | TCCACCACAGTTGCTGACTC | |
| CD4 | ||
| Forward | ACACACCTGTGCAAGAAGCA | 69 |
| Reverse | GCTCTTGTTGGTTGGGAATC | |
| CD8 | ||
| Forward | TTACATCTGGGCACCCTTG | 132 |
| Reverse | TTGCCTTCCTGTCTGACTAGC | |
| NKR-P1A | ||
| Forward | GGCTTGGCATGAGTCACC | 75 |
| Reverse | TTCAGAGCCAACCTGTGTGA | |
| β-actin | ||
| Forward | GGTCATCACTATTGGCAACG | 133 |
| Reverse | ACGGATGTCAACGTCACACT | |
| Conventional RT-PCR | ||
| AMHR2 | ||
| Forward | GTATCCGCTGCCTCTACAGC | 193 |
| Reverse | CAGAAGTCAGTGCCACAGGA | |
| β-actin | ||
| Forward | GGTCATCACTATTGGCAACG | 133 |
| Reverse | ACGGATGTCAACGTCACACT |