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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Oncogene. 2015 May 18;35(7):919–928. doi: 10.1038/onc.2015.146

Figure 1. MALT1 is selectively involved in EGFR-induced NF-κB activation.

Figure 1

(a) A431 cells with a MALT1 knockdown (shMALT1) and control cells (shCtl) were stimulated with EGF (100ng/ml), PMA and Ionomycin (50ng/ml; 100ng/ml) or TNFα (10ng/ml) for indicated periods. NF-κB activation and Oct-1 (loading control) levels were determined by the gel shift assay. (b) MEFs from Malt1+/− and Malt1−/− embryos were isolated. Early-passage (P1) MEFs were stimulated with PMA and Ionomycin (50ng/ml; 100ng/ml) or TNFα (10ng/ml) for indicated periods. NF-κB activation and Oct-1 levels were determined by the gel shift assay. (c) Nuclear lysates from A431 and A431-shMATL1 cells were analyzed by immunoblotting using indicated antibodies. (d) shMALT1 and shGFP (control) A431 cells were stimulated with EGF (100ng/ml) for indicated periods. Cell lysates were analyzed by immunoblotting using indicated antibodies.