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. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: Cancer Res. 2015 Sep 17;75(22):4863–4875. doi: 10.1158/0008-5472.CAN-14-2345

Figure 3.

Figure 3

Src regulates glucose uptake and GLUT1 expression. A & B, Glucose uptake in vector control, 10A.B2, and 12A.B2 cells treated with vehicle or saracatinib. C & D, Glucose uptake in vector control, ctrl.shRNA-, and Src.sh-transfected 10A.B2 and 12A.B2 cells. E&F, Immunoblots and respective quantifications showing GLUT1 and Src expression in vector-, Src wt-, and SrcY527F-expressing MCF10A and MCF12A cells in 3D culture. G&H, Immunoblots and respective quantifications showing GLUT1, phospho-Src-Y416, and Src in vector control, 10A.B2, 12A.B2, ctrl.shRNA-, and Src.sh-clones of 10A.B2 and 12A.B2 cells grown in 3D culture. I&J, qRT-PCR showing GLUT1 in vector control, 10A.B2, 12A.B2, ctrl.shRNA and Src.sh clones of 10A.B2 and 12A.B2 cells grown in 3D culture. K, Immunoblot showing GLUT1 in vehicle- and saracatinib-treated 10A.B2 and 12A.B2 cells grown in 3D culture.